An Integrated Microfluidic Device for Large Scale in Situ Click Chemistry Screening

Summary:
An integrated microfluidic device has been developed to perform 1024 in situ click chemistry reactions in parallel using the bovine carbonic anhydrous II (bCAII) click chemistry system as a proof-of-concept study and a rapid hit identification approach using SPE purification and electrospray-ionization mass spectrometry, multiple reaction monitoring (MRM) analysis, all of which improves the sensitivity and throughput of the downstream analysis.

Scientists:
Yanju Wang, Wei-Yu Lin, Kan Liu, Rachel J. Lin, Matthias Selke, Hartmuth C. Kolb, Nangang Zhang, Xing-Zhong Zhao, Michael E. Phelps, Clifton K. F. Shen, Kym F. Faull and Hsian-Rong Tseng.

Institutions:
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 570 Westwood Plaza, building 114, Los Angeles, CA 90095, USA. E-mail: hrtseng@mednet.ucla.edu

Pasarow Mass Spectrometry Laboratory, Department of Psychiatry & Biobehavioral Sciences and the Semel Institute for Neuroscience and Human Behavior, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA. E-mail: faull@chem.ucla.edu

Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, CA 90032

Siemens Medical Solutions USA, Inc., 6140, Bristol Parkway, Culver City, CA 90230, USA

Department of Physics, School of Physics, Center of Nanoscience and Nanotechnology, Wuhan University, Wuhan, 430072, China

Feed:
Received 14th April 2009 , Accepted 11th June 2009.

First published on the web 17th June 2009.